Endogenous Thiadiazabicyclo Glutathione-adduct invention
Endogenous
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims priority of U.S. Provisional Application Ser. No. 60/853,714 filed Oct. 23, 2006, which is incorporated herein by reference in its entirety.
GOVERNMENT INTERESTThis invention was supported, in part, by Grant Numbers CA 91016, CA95586, HL70128, and ES013508 from the NIH. The government may have certain rights in the invention.
FIELD OF THE INVENTIONThe invention is directed to mediators of apoptosis. Specifically, the invention relates to thiadiazabicyclo-4-oxo-2(E)-nonenal-Glutathione adduct as a novel agent for inducing apoptosis in cells depleted of GSH.
BACKGROUND OF THE INVENTIONEndothelial dysfunction is considered to be the earliest event in atherogenesis. Oxidative stress, inflammation, and apoptosis play critical roles in its progression and onset. Lipid peroxidation, which occurs during oxidative stress, results in the formation of lipid hydroperoxide-derived bifunctional electrophiles such as 4-hydroxy-2(E)-nonenal (HNE) that induce apoptosis.
Conversely, cancer cells undergo changes which abrogate apoptosis, resulting in immortalization.
There is a need in the art for finding compounds and markers that will both cause apoptosis and will mark the progression of apoptosis due to pathological processes in the cell.
SUMMARY OF THE INVENTIONIn one embodiment, the invention provides a composition comprising Thiadiazabicyclo-4-oxo-2(E)-nonenal-Glutathione (TOG).
In another embodiment, the invention provides a method of inducing apoptosis in a cell comprising the step of contacting said cell with Thiadiazabicyclo-4-oxo-2(E)-nonenal-Glutathione (TOG), thereby inducing apoptosis in a cell.
In one embodiment, the invention provides use of Thiadiazabicyclo-4-oxo-2(E)-nonenal-Glutathione (TOG) in a composition for the treatment of hypeproliferative disease in a subject in need thereof
BRIEF DESCRIPTION OF THE DRAWINGSThe invention will be better understood from a reading of the following detailed description taken in conjunction with the drawings in which like reference designators are used to designate like elements, and in which:
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Extraction Of Nonenal - Bookshelf
Coffee flavor chemistry
2-Nonenal without specification of stereochemistry was identified in green ... of 280 ppb (simultaneous distillation/extraction, column chromatography, ...Journal of the Institute of Brewing
The PLS regression coefficients contribution of £-2-nonenal suggests that ... beer by stir bar sorptive extraction with in-situ derivatizau'on and thermal ...International journal of environmental analytical chemistry
A method is described for isolation of E-2-nonenal from beer by solid phase extraction. After extraction E-2-nonenal is derivatized with dansylhydrazine and ...Volatile compounds in foods and beverages
Subsequent studies led to the identification of (Z)-3-nonenal and (IZ,/2)-3 ... The isolation of an enzyme system from cucumber that catalyzes the removal ...Eighteenth International Seaweed Symposium, Proceedings of the Eighteenth International Seaweed Symposium Held in Bergen, Norway, 20 - 25 June 2004
... phase micro extraction (SPME) techniques. ... 3Z-nonenal and 2E-nonenal) from arachidonic acid In higher plants, C6 and C9 aldehydes are formed from C18 ...Casual Walkthroughs Directory
Evaluation of PDMS-Based Extraction Techniques and GC-TOFMS ...
Evaluation of PDMS-Based Extraction Techniques. and GC-TOFMS for the ... (Z)-2-nonenal, a potential cause of stale flavor in beer. This figure shows the non ...
Evaluation of PDMS-based Extraction Techniques and GC-TOFMS ...
of the PDMS extraction techniques and replicate analyses of beer ... trations of t-2-nonenal and other aldehydes. The most significant changes in the light ...
Solid phase extraction and liquid chromatography-tandem mass ...
Solid phase extraction and liquid chromatography-tandem mass spectrometry for the evaluation of 4-hydroxy-2-nonenal in pork products. Auteur(s) / Author(s) ...
Imgenex citations of : Nuclear Extraction kit (10081K)
Imgenex citations of : Nuclear Extraction kit (10081K) 1. Molecular cloning and ... Mechanisms of 4-hydroxy-2-nonenal induced pro- and anti-apoptotic ...
Ciência e Tecnologia de Alimentos - (E)-2-Nonenal ...
(E)-2-nonenal is considered an important off-flavor of beer, related ... results were found with 15 minutes of equilibrium and 90 minutes of extraction at 50 °C. ...